Biotechnology

Biotechnology #1. a) The Use of a Bacterial Plasmid to toller and Sequence a Human Gene The process begins with limit endonucleases scanning and binding to double-stranded DNA at specific base-pair sequences, the information sites, in a predictable manner. The restriction sites are normally 4 to 8 base pairs long and are characterized by the palindromic sequences, with both strands having the same sequence when read in resister direction.

After the restriction endonuclease binds, it starts to disrupt, using hydrolysis, the phosphodiester bonds between neighbor nucleotides, cause the H-bonds between base pairs in the deoxidiseting region to be broken. This cuts the original double-stranded DNA strand, producing two DNA fragments, which may dissent for different restriction endonucleases, depending on where the phosphodiester bond is broken when cut by the endonuclease. This process can produce either girdle ends (where ends of the DNA fragment are fully paired with no overhangs),...If you want to get a full essay, order it on our website: Orderessay

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